Study Set Content:
What protein complex facilitates the recruitment of the kinetochore proteins to the centromeric site?
CCAN OR ICEN, CCAN, ICEN
What is the repeating unit of the chromatin?
NUCLEOSOME
The histone octamer is composed of how many histone protein sub-units?
8
What are the histone protein sub-units that make up the octameric protein? (Hint: there are 2 of each). Separate with a comma, space. In CAPITAL letters.
H2A, H2B, H3, H4
The H2A histone variant specific to humans and are expressed in the testes and brain. WRITE THE CORRECT "case" of each letter. Wrong case, wrong answer.
H2A.Bbd
A post-translational modification that involves the covalent attachment of small ubiquitin- like modifier proteins to other proteins to modify their function. (CAPITAL letter answers).
SUMOYLATION
What are the 2 models proposed for the chromatin secondary structure? ____________ AND ____________.
SOLENOID MODEL AND ZIGZAG MODEL
A diverse group of nuclear proteins that prevent the aggregation of folded histones with DNA during the assembly of nucleosomes. (CAPITAL letter answer)
HISTONE CHAPERONES
Chemical modifications added post-translationally (and reversibly) to many histone amino acid side chains by highly specific enzymes. (Do not write the acronym. CAPITAL letter.)
POST-TRANSLATIONAL MODIFICATIONS
Abundant nuclear proteins that interact with nucleosomes and influence the three-dimensional arrangement of nucleosomal arrays. (Do not write the acronym. CAPITAL letter.)
ARCHITECTURAL CHROMATIN PROTEINS
POST-TRANSLATIONAL MODIFICATIONS
UBIQUITYLATION
Which of the following examples is NOT a case of Euploidy?
A metafemale fruit fly (D. melanogaster) with 2n = 6 + XXY
What is the general consequence of a merotelic attachment of kinetochore to both spindle poles?
ANEUPLOIDY
The bi-oriented attachment of sister kinetochores to microtubules emanating from opposite centrosomes is called:
AMPHITELY
For unresolved merotelic attachments that lags at the cleavage furrow, the consequence would be
STRUCTURAL ANEUPLOIDY
Damage of the enzyme telomerase can lead to uncapped chromosomes that can fuse with another chromosome. What mechanism of DNA repair is responsible for this? Use the ABBREVIATION only.
NHEJ
What is the identity of the molecular "glue"
COHESIN COMPLEX
Recall the lecture in Prelims about segregation of chromatids. What enzyme is responsible for the hydrolysis or breakdown of the molecular glue described in number 5?
SEPARASE
56. TETRAPLOIDY CAN RESULT FROM: SI. Defects in centrosome duplication SII. Endoreplication
BOTH S1 AND SII ARE CORRECT
What are the causes of structural aneuploidy. Name 3. Put a space and a comma after each.
NON-BALANCED TRANSLOCATION, DELETION, DUPLICATION
