Appropriate steps for decontamination and disinfection of spills involving biohazard materials.

1) Put on heavy weight puncture-resistant utility gloves, a gown, and if necessary water-impermeable shoes.

2) Remove ang sharp broken objects without touching them by using rigid sheets of cardboard to scrape them up. Discard cardboard with broken objects in puncture-resistant biohazard container or receptacles.

3) Absorb the spill with disposable absorbent material (e.g. paper towels, gauze pads or tissue paper wipes). Dispose of contaminated materials in biohazardous waste bin.

4) Clean spill site of all visible spilled materials using any aqueous household detergent. This dilutes spilled materials, lysis erythrocytes and removes proteins.

5) Disinfect spill site using intermediate to high level hospital disinfectant such as a dilution of household bleach. Flood spill site or wipe down with disposable towels soaked in disinfectants to make site glistening wet. Phenolic disinfectants are acceptable for use on laboratory instruments, floors or countertops.

6) Absorb disinfectant with disposable material or allow disinfectant to dry.

7) Rinse all spill site with water to remove odors, dry spill site to prevent slipping. 8) Dispose of all contaminated materials used in cleaning process in a biohazard container.

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Hematology Lab (lesson 1)

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