Study Set Content:
Blood agar type
enriched
Chocolate agar
Enriched
Modified Thayer Martin Aga
Selective
Cary and Blair Agar
Transport Medium
MacConkey Agar
Selective and Differential
Lowenstein Jensen Agar
Selective
Triple Sugar Iron Agar
Differential
Bordet – Gengou Agar
Selective
Thioglycollate Broth
Anaerobic media
Xylose Lysine Desoxycholate Agar
Selective
Eosin Methylene Blue Agar selective for gram
Selective
SS Agar -
Selective Differential
Saboraud’s dextrose agar
Selective
is a general purpose, enriched medium often used to grow fastidious organisms and to differentiate bacteria based on their hemolytic properties. In the U.S., is usually prepared from tryptic soy agar or Columbia agar base with 5% sheep blood.
Blood Agar
s a nonselective, enriched medium used for the isolation and identification of fastidious pathogens. Chocolate agar is prepared by heating blood agar, which in turn ruptures the red blood cell (RBC) and releases nutrients that aid in the growth of fastidious bacteria, most notably Haemophilus and Neisseria species. The name is derived from the fact that the lysis of RBC gives the medium a chocolate-brown color.
Chocolate Agar
used for the isolation of gonococci (Neisseria gonorrheae) from specimens containing a mixed flora of bacteria and/or fungi e.g. urogenital specimen. is a GC agar base containing vancomycin, colistin, nystatin, and trimethoprim lactate (VCNT). Selective isolation of Neisseria gonorrhoeae is achieved with suppression of most other gram-negative diplococci, gram-negative bacilli, gram-positive organisms, and yeast.
Modified Thayer Martin Agar
is a selective and differential bacterial culture media. MacConkey medium is selective for gram-negative bacteria and differentiates the gram-negative bacteria based on lactose metabolism.
MacConkey Agar
is used for the differentiation of gram-negative enteric bacilli based on carbohydrate fermentation and the production of hydrogen sulphide.
Triple Sugar Iron Agar
emi-solid, nonnutritive medium used for the collection and preservation of microbiological specimens. The minimal nutrients in the medium facilitate the survival of organisms without multiplication. The semisolid consistency provides ease of transport, and the prepared medium can be stored for up to 1 year after preparation at room temperature.
Cary and Blair Agar
is the selective medium which is used for the cultivation and isolation of Mycobacterium species. It was developed by Lowenstein who incorporated congo red and malachite green to inhibit unwanted bacteria.
Lowenstein Jensen Agar
