he media contain sucrose, which is utilized by ferment microbes and helps to distinguish them from non-ferment microorganisms. Based on this characteristic, different colored bacterial colonies are formed on the media that help to identify and distinguish them from each other. For example, V. cholerae ferment the sucrose and form slightly flattened yellow colonies having opaque centers and translucent peripheries. Whereas, V. parahaemolyticus can’t ferment the sucrose and forms green to blue-green colonies