Study Set Content:
According to this hypothesis, the enzyme's active site is not a rigid structure, but rather a flexible and dynamic structure that changes its shape slightly to accommodate the substrate.
Induced Fit Model
The binding of the substrate to the enzyme's active site induces a ()in the enzyme, leading to a better fit between the substrate and the active site.
conformational change
This change may involve the () or loops in the enzyme's structure.
rearrangement of side chains
enzyme + substrate entering active site
enzyme/substrate complex
enzyme/product complex
enzyme + product leaving active sit
Substrate entering active site of enzyme
Enzyme/ substrate complex
Enzyme/products complex
Products leaving active site of enzyme
Factors that affect enzyme activity
Temperature
pH
- All enzymes work within limited temperature ranges. - The optimal temperature range for most plant enzymes is
35°C and 40°C.
- Enzymes cannot tolerate high temperatures. Temperature above the optimal range destroys the enzymes, whereas their activity is negligible at
0°C.
- Plant enzymes work in a very broad pHrange, () which coincides very nicely with the humangastrointestinal tract.
3.0 to 9.0,
Plant enzymes work in both the
stomach and intestines
Detection of Dehydrogenase Reagent
Sliced potato+Cold water + Triphenyl tetraxolium chloride(0.2%)
Detection of Dehydrogenase Reagent
Sliced potato+Cold water + Triphenyl tetraxolium chloride(0.2%)
Result
Red coloration
Detection of Dehydrogenase Reagent
Sliced potato+Cold water + Methylene blue(0.1%)
Detection of Dehydrogenase Reagent
Sliced potato+Cold water + Methylene blue(0.1%)
Result
Blue color is discharged
