Measurement of the attenuation of a beam of
light after it passes through a sample or after
reflection from a sample surface.
UV-Vis
refers to spectroscopic techniques that measure the
absorption of radiation, as a function of
frequency or wavelength, due to its
interaction with a sample
Absorption spectroscopy
encompasses
the wavelength regions;
Absorption spectrometry
(200–380 nm)
ultraviolet
(380–780 nm)
visible
(780 nm to 2.5 m) and
near infrared
(2.5–40 m).
infrared
commonly treated as a wave phenomenon,
characterized by a wavelength or frequency.
Electromagnetic radiation
is the number of wave cycles that
travel past a fixed point per unit of time, and
is usually given in cycles per second, or hertz
(Hz).
Frequency
is the distance between
adjacent peaks , and may be designated in
meters, centimeters or nanometers (10-9
meters).
Wavelength
DISPERSIVE ELEMENT
EXIT SLIT
SAMPLE
DETECTOR
MONOCHROMATOR
Vs = Vu / Vo
Sedimentation Volume
Redispersibility – the amount of the
force necessary to redisperse
particles, product is subject to
shaking for minimum of
10 spm
determines the repulsive
forces between particles
Zeta Potential