Study Set Content:
UV region (190 nm to 400 nm), a ____ is commonly used due to its broad spectral output and high intensity in this range.
Deuterium lamp
Visible region (400 nm to 800 nm), ____ (Iodine and bromide) lamps are preferred for their continuous spectrum.
Tungsten or Halogen
(190-800 nm) – High-intensity light source used for both UV and Visible ranges.
Xenon lamp
In contrast to tungsten and halogen lamps, it is less stable and more costly.
Xenon lamp
These lamps provide stable and consistent light output necessary for
accurate measurements.
Once the light is emitted from the source, it passes through the monochromator, which acts as a
wavelength selector
The monochromator can be either a
prism or a diffraction grating
It disperses the polychromatic light into its component wavelengths, allowing only a narrow range of wavelengths to pass through at any given time.
Monochromator
By rotating the grating or prism, different wavelengths can be selected for
analysis.
defines narrow beam of radiation from source
Entrance slit
collimates the lights
Collimating lens (polished surface)
disperses the light into specific wavelength.
Prism (make-quartz)
captures the dispersed light sharpens the same to the sample cuvette via exit slit
Focusing lens
allows the corrected wavelength of light to the sample cuvette
Exit slit
TYPES OF MONOCHROMATORS
Prism Monochromator
Grating Monochromator
Filter Monochromator
Utilizes a prism to separate polychromatic light into its component wavelengths. The angle of the prism can be adjusted to select the desired wavelength.
Prism Monochromator
While simple, prism monochromators may have limitations in
spectral resolution.
is the choice of material of UV Spectrum
Quartz or Fused Silica
