Study Set Content:
is used to detect cholesterol in a solution.
Salkowski test
When potassium
bisulfate is heated with a
fat,
hydrolysis occurs,
When potassium
bisulfate is heated with a
fat, hydrolysis occurs, and the glycerol produced is
dehydrated to form
to form acrolein (CH2=CHCH0).
has a characteristic sharp irritating odor
Acrolein
cholesterol is dissolved in
chloroform
cholesterol is dissolved in
chloroform then what are added
acetic
anhydride and sulfuric acid
A color change from pink which gradually turns into (blank) is observed
deep green
It is an important test used to detect cholesterol
depending on the colors (distinct and clear colors)
that yield from the reaction of
cholesterol with concentrated
sulfuric acid.
Salkowski test
Salkowski test
the top layer is
red
Salkowski test
and the
bottom layer is
green
It is the process that
stabilizes the water and oil
emulsion by using the
emulsifying agents
Emulsification Test
emulsify the lipid, after
which the lipids appear as
the tiny droplets
suspended in the solution.
Emulsifying agents
is also
used to detect the
presence of glycerol.
Dichromate test
Dichromate test is also
used to detect the
presence of glycerol. It
is based on the principle
of an
oxidation
reaction.
In this, glycerol and
dichromate ions react to
give a
brown color to
the solution.
Then, the chromic ions oxidize the
glycerol and reduce into chromous ions by giving a
blue colour
Then, the chromic ions oxidize the
glycerol and reduce into chromous ions by giving a
blue colour to the solution in the presence of
nitric
acid.
Readily miscible in
nonpolar solvents
Solubility Test
nonpolar solvents
(hexane, chloroform,
carbon tetrachloride,
benzene,
DCM)
The appearance of translucent spot
confirms the presence of fats in the
given sample.
Grease Spot/Translucent Spot
