Study Set Content:
he plate would be placed into a shallow pool of "development" solvent and allowed to move up the plate by
capillary action.
Once complete, remove and allow the solvent to
evaporate
Colored compounds would be shown as distinct spots.
Visualization -
can be used to visualize colorless compounds.
UV light or lodine vapor
Why must the spot be applied to the TLC plate above the level of development solvent?
So that the sample would not dissolve in the development solvent
What will be the result of adding too much sample to the TLC plate?
The sample would streak or trail along the plate.
In a TLC analysis of analgesics, what would be the result if a solvent of too low polarity is used to develop the plate?
The components will not separate or move enough, resulting to a very small Rf value
Ethyl ester or
Para-Aminobenzoic Acid
The preparation is done by
Esterification
is an organic reaction which is employed to convert carboxylic acids in the presence of excess alcohol and a strong acid catalyst to give an ester as the final product.
Fischer Esterification
This ester is formed along with
water
is generally well tolerated and non-toxic when applied topically as recommended.
Benzocaine
However, there have been reports of serious, life-threatening adverse effects (e.g., () ) with overapplication of topical products or when applying topical products that contain high concentrations of benzocaine to the skin.
seizures, coma, irregular heartbeat, respiratory depression
To treat a variety of
pain-related conditions.
