Study Set Content:
Applications: To classify enteric microorganism +
Klebsiella (+) Enterobacter (+) Salmonella (+
-
E.coli
Urease test Principle/Inference Hydrolysis of
urea (urease)
Hydrolysis of urea (urease) and production of an
alkaline product (NH3)
Urea test indicator
Phenol Red
Neutral
orange
Alkaline
pink
acid
yellow
POSITIVE
pink (alkaline)
Urea test negative
No color change
Urea test Products formed:
NH3
CO2
Gelatin liquefaction/ Hydrolysis test principle
Detects presence of
 proteases
Detects presence of proteases for hydrolysis of
gelatin
Detects presence of proteases for hydrolysis of gelatin or liquefaction o
solid gelatin medium
Gelatin liquefaction/ Hydrolysis test positve
Solid medium to semisolid/liquid medium
Gelatin liquefaction/ Hydrolysis test NEGATIVE
1. No change
Applications: +) positive
S. aureus
-) negative
E. coli –
IMViC reactions
Indole Ring Test, Methyl Red Test, Vogues-Proskaur Test- Citrate utilization test
IMViC reactions identification of family
Enterobacteriaceae
