Study Set Content:
Greek chroma –
Color
Graphein –
“Writing”
Russian Botanist, referred to as Father of Chromatography.
Michael Tswet
Chromatography is usually based on principle of partition of solute between two phases. Consists mostly of a
Mobile Phase and Stationary Phase.
Refers to the mixture of the substances to be separated dissolved in a liquid or a gas
The Mobile Phase
– A porous solid matrix through which the sample contained in the mobile phase percolates.
Stationary Phase
Chromatographic Methods can be classified in three different ways:
1. Based on the shape of chromatographic beds. example:
2. Based on the physical state of mobile and stationary phase, for example:
3. Based on the mechanism of separation, ex.
Based on shape of chromatographic beds. example
Planar and Column
Based on the physical state of mobile and stationary phase, example:
Gas and Liquid
Based on mechanism of separation, ex
. Ion-exchange, partition, affinity and adsorption
Planar and Column
Under Planar
Paper and Thin Layer
Column
Gas and Liquid
stationary phase is present on a plane.
Planar Chromatography
Liquid Partition.
Paper Chromatography –
Planar Chromatography can either be
paper or thin layer chromatography
Planar Chromatography t is also known as
Open Bed Chromatography.
Stationary phase is consists of a thin layer of adsorbent material, usually silica gel, aluminium oxide or cellulose immobilize onto a flat carrier sheet.
Thin Layer Chromatography
The stationary bed is within the tube. And it may be pure silica or polymer or may be coated onto, chemically bonded to, support particles.
Column Chromatography
When the Stationary phase in LC consists of small diameter particles, the technique is
High Performance Liquid Chromatography (HPLC)
