Study Set Content:
A simple and accurate means for determination and investigation of structure in macro, semi micro and micro analysis of expensive and non-duplicable samples.
POLARIMETRIC METHOD
Polarimetry measures the rotation of ____as it passes through an optically active fluid.
polarized light
The measured rotation can be used to calculate the value of solution concentrations; especially substances such as
sugars, peptides and volatile oils.
to measure the rotation angle, and sample tubes
polarized light source, an analyzer, a graduated circle
The polarized light passes through the sample tube and exhibits angular rotation to the ()
left (-) or right (+).
On the side opposite the polarizer is the
analyzer.
analyzer. Using optics, visual fields are manually adjusted by the user to measure the
optical rotation angle
The sodium light with a wavelength of () is linearly polarized by means of a
589nm, polarizer.
This light then passes through the sample solution to be observed through an
analyzer.
Optically active substance rotates the
polarization plane
By measuring the____by means of the analyzer the concentration of the solution can now be calculated
rotational angle
