Study Set Content:
is faster, more sensitive and has more precision than a conventional spectrometer due to the photo diode-array detection system.
Diode Array Spectrophotometer
APPLICATIONS OF UV-VIS
Determination of the pKa values of some drugs.
Determination of partition coefficients and solubilities of drugs.
It is used for characterizing aromatic compounds and conjugate olefins.
It can be used to find out molar concentration of the solute under study.
It is one of the important methods to detect impurities in organic solvents.
Quantification of Active Pharmaceutical Ingredients
Assay of Impurities
Determination of Drug Release
Protein Quantification
Nucleic Acid Quantification
Colorimetric Assay
ASSAY OF UV-VIS
1. Assay of Furosemide in Tablet form
2. Assay of cyclizine lactate in injection
3. Assay of Penicillin by derivatization
4. Assay of adrenaline in lidocaine (lignocaine) adrenaline injection
Analyzes how molecules interact with infrared light.
IR SPECTROSCOPY
Molecules absorb specific frequencies of infrared light, providing insights into their
structure and functional groups.
A powerful analytical technique that deals with the interaction of infrared radiation with matter through absorption, emission, and reflection.
IR SPECTROSCOPY
This technique has widespread applications in chemistry, biology, materials science, and other fields
IR SPECTROSCOPY
causes the excitation of the vibrations of covalent bonds within that molecule. These vibrations include stretching and bending modest.
IR radiation
In practice, it is the____ that are IR "active" and whose excitation can be observed in an IR spectrum.
polar covalent bonds
It is widely used to study and identify chemical substances or functional groups in solid, liquid, or gaseous forms.
IR SPECTROSCOPY
deals with the infrared region of the electromagnetic spectrum, which is. light having a longer wavelength and a lower frequency than visible light. It generally refers to the analysis of the interaction of a molecule with infrared light.
IR spectroscopy
Infra-red spectrometer produces
infrared spectrum.
Provides a complex fingerprint which is
unique to the compound being examined
STRENGTH
Rarely used as a quantitative technique
because of relative difficulty in sample
preparation and the complexity of
spectra.
LIMITATIONS
Usually, can only detect gross impurities
in samples.
LIMITATIONS
Computer control of instruments means
that matching of the spectrum of a
compound to its standard fingerprint can
now be readily carried out
STRENGTH
Sample preparation requires a degree of
skill, particularly when potassium bromide
(KBr) discs are being prepared.
LIMITATIONS
The technique is lacking in robustness
since sample handling can have an effect
on the spectrum obtained and thus care
has to be taken in sample processing
LIMITATIONS
