Study Set Content:
Instruments have:
•
radiant power source
•
radiant energy dispersing device
•
a sample compartments
Spectrophotometer – Sample Cells
UV Spectrophotometer
Quartz (crystalline silica)
Visible Spectrophotometer
Glass
IR Spectrophotometer
NaCl
Spectrofluorometer
Quartz
Spectrophotometer – Light Source
•
UV Spectrophotometer
Hydrogen Gas Lamp, Mercury Lamp, Deuterium
Visible Spectrophotometer
Tungsten Lamp
IR Spectrophotometer
Carborundum (SIC)
Spectrofluorometer
Xenon lamp, Alkaline halide lamp
TYPES OF SPECTROPHOTOMETERS
Single Beam Spectrophotometer
Double Beam Spectrophotometer
Diode Array Spectrophotometer
are the most basic type of UV/VIS spectrophotometer.
Single-beam spectrophotometers
feature two separate light beams: one passes through the sample, while the other passes through a reference solution simultaneously.
Double-beam spectrophotometers
They consist of a single light beam that passes through the sample and a reference solution (often a blank solvent) before reaching the detector
Single Beam Spectrophotometer
The absorbance of the sample is determined by comparing its intensity to that of the reference solution.
Single Beam Spectrophotometer
The absorbance of the sample is determined by comparing the intensity of the two beams after they pass through their respective paths.
Double Beam Spectrophotometer
are simple, cost-effective, and suitable for routine measurements where high accuracy is not critical.
Single-beam spectrophotometers
single- beam, microprocessor-controlled spectrophotometer
Diode Array Spectrophotometer
DIODE ARRAY SPECTROPHOTOMETER Visible/UV - range of () to () nm with () nm resolution
190- 820, 2
offer improved accuracy and stability compared to single-beam instruments, as they automatically compensate for fluctuations in light source intensity and detector sensitivity.
Double-beam spectrophotometers
It is used when speed of measurement is essential
Diode Array Spectrophotometer
