It determines in vitro sensitization
IAT (Indirect Antiglobulin Test)
It is employed in crossmatching, antibody determination, antibody identification, red cell antigen phenotyping.
IAT (Indirect Antiglobulin Test)
Example of Antiglobulin test
D^U Test
we mix serum of the patient with the red cells of the donor, re-creating the scenario in vitro.
Major Crossmatching
No reaction =
With reactions =
No reaction = compatible
With reactions = find another donor
involves with the aggregation of soluble antigens.
Precipitation
in the cell membranes results to inability of the antibodies bind with Ags
Small or buried deeply antigenic sites
antigens are naturally present on the rbc surface (ABO blood typing); forward typing
Hemagglutination