Study Set Content:
Then, sodium sulfide reacts with the
lead acetate
Then, sodium sulfide reacts with the lead acetate and undergo precipitation reaction by forming
black precipitates as lead sulfide.
is a general test for proteins
Biuret Test
Biuret Test is a general test for proteins. When a protein reacts with
copper (II) sulfate (blue)
Biuret Test is a general test for proteins. When a protein reacts with copper (II) sulfate (blue), the positive test is the formation of a
violet-colored complex.
Biuret test is based on binding of
copper ions
Biuret test is based on binding of copper ions to
peptide bonds
Biuret test is based on binding of copper ions to peptide bonds under
alkaline condition
Millon's test
Xanthoproteic test
Pauly’s Diazo Test
Pauly’s Diazo Test
Hopkins-Cole Test
Lead Sulfide Test
Biuret Test
involves the disruption and possible destruction of both the secondary and tertiary structures.
Protein denaturation
Since denaturation reactions are not strong enough to break the (blank) the primary structure (sequence of amino acids) remains the same after a denaturation process.
peptide bonds
Denaturation disrupts the normal
alpha-helix and beta sheets
Denaturation disrupts the normal alpha-helix and beta sheets in a protein and uncoils it into a
random shape.
occurs because the bonding interactions responsible for the secondary structure (hydrogen bonds to amides) and tertiary structure are disrupted.
Denaturation
