Study Set Content:
Without this enzyme it can no longer make new cross-links, all the while continuing to make enzymes that hydrolyze(break down) these links.
DD-transpeptidase.
This will cause (blank) in the cell wall to form and eventually force the bacteria to shed most if not all of its wall
holes
What factors affect enzyme activity?
Enzyme and substrate concentration
Temperature:
• pH
the higher the enzyme and substrate concentrations, the higher the enzyme activity. At sufficiently high substrate concentration, however, a saturation peak is reached. After this point, increasing substrate concentration no longer increases the reaction rate
Enzyme and substrate concentration:
: in uncatalyzed reactions, the rate increases with
increased temperature
in uncatalyzed reactions, the rate increases with increased temperature, however, in enzyme-catalyzed reactions, increasing the temperature will inactivate the enzyme. Optimum temperature is around
37°C.
pH-related effects resemble those observed when the temperature
changes
pH-related effects resemble those observed when the temperature changes. Each enzyme operates best at a certain
pH
pH-related effects resemble those observed when the temperature changes. Each enzyme operates best at a certain pH. Extreme pH values will
denature enzymes irreversibly.
pH optimum
Lipase
8.0
Lipase(stomach)
4-5
Lipase(castor oil)
4.7
Pepsin
1.5-1.6
Trypsin
7.8-8.7
Urease
7
Invertase
4.5
6.1-6.8
Maltase
Amylase(pancres)
6.7-7.0
Amylase(malt)
4.6-5.2
Catalase
7
