Study Set Content:
ethylene or propylene oxide gas
Gas sterilization
alters metabolism of cell
Gas sterilization
for heat-sensitive prep
Filtration
for powders
Dry heat
For heat and moisture sensitive prep
Gas sterilization
Must allow desorption
Gas sterilization
destroy all living organisms and their spores OR their complete removal from the preparation
Sterilization
150°C to 170°C NLT 2 hours.
Dry Heat
Using gamma rays and cathode rays )
Ionizing radiation
Ionizing radiation
25-40 kGy
Complete and irreversible destruction of cell components
Ionizing radiation
- spores of Bacillus stearothermophilus
Steam and gas sterilization
spores of B. pumilus, B. stearothermophilus, B. subtilis
Ionizing radiation
spores of Bacillus subtilis
Dry heat
is a characterized preparation of specific microorganisms resistant to a particular sterilization process.
biologic indicator
Uses rabbits Checks the individual rise in temperature
USP Pyrogen Testing
aka Bacterial Endotoxins Test USP uses LAL (Limulus amebocyte lysate) from horseshoe crab
USP Endotoxin Testing
USP Pyrogen Testing pass
NMT 3 out of 8 showed + 0.5 °C or more / Sum of temperature rise is NMT 3.3 °C
USP Endotoxin Testing Pass
No gel clot is formed
Clotting means
with endotoxin
