Study Set Content:
C. TSI Indicator
Phenol Red.
TSI positive
A. yellow slant
B. Yellow butt
C. Cracks
. yellow slan means
glucose fermenter
Cracks means
Gas production – displacement of the media)
TSI negative
A. Red slant
B. H2S(black pp)
C. Yellow Butt
D. No cracks
TSI products formed
H2S/Fe2S – black ppt.
Lysine Iron Agar Slant
Principle/Inference
Deaminate/ decarboxylate lysine
. Lysine deamination is an
aerobic process
Lysine deamination is an aerobic process which occurs on the (blank) of the media
slant
Lysine decarboxylation is an
anaerobic process
Lysine decarboxylation is an anaerobic process which occurs in the (blank)of the media.
butt
. Lysine Iron Agar Slant components
glucose, ferric ammonium citrate, sodium thiosulfate and bromocresol purple
Lysine Iron Agar Slant pH indicator
bromocresol purple
Below 5.2
yellow
Above 6.8 =
purple
Deaminase ferric ammonium citrate and coenzyme
Burgundy
No deamination
purple
Lysine decarboxylase >
cadaverine
Glucose fermentation Lysine decarboxylase > cadaverine >
neutralize acids
Lysine decarboxylase > cadaverine > neutralize acids =
purple
