Study Set Content:
purple means
alkaline
No decarboxylase =
yellow
yellow means
acidic
, Alkaline slant/alkaline butt
(K/K)
Alkaline slant/alkaline butt, H2S positive
(K/K H2S+).
Alkaline slant/acid butt
K/A).
Red slant/acid butt
(R/A).
Alkaline slant/alkaline butt
-/+
, Alkaline slant/alkaline butt, H2S positive
-/+ with H2S
, Alkaline slant/acid butt
-/-
Red slant/acid butt
+/-
Lysine Iron Agar Slant
Applications: >differentiate
gram- bacilli
Applications: >differentiate
lactose fermenting to non-lactose fermenting salmonella
Principle/Inference V E. INDOLE
Principle: Certain bacteria possess the enzyme
tryptophanase
Indole positive
Indole ring formation
NEGATIVE
No ring formation
Applications: To differentiate:
E. coli, Enterobacter, Klebsiella spp, Bacillus, Salmonella spp.
Manufacture/production of
indole containing products
Principle/Inference
Methyl Red Test
Glucose fermenters produce (by product – pyruvic acid) resulting to change in the
pH
indicator
Methyl Red
