Study Set Content:
Consistency
Oily Viscous
Solubility Test for Lipids Insoluble
Distilled Water
Dil. NaOH
Dil. HCl
Alcohol
Partially Soluble
Acetone
Soluble
Chloroform
Soluble
Insoluble / immiscible in
polar solvents
Soluble / miscible in
non-polar / organic solvents
Translucent Spot Test for Lipids: ➢ Positive result
Formation of greasy or translucent spot in the paper confirms the presence of fat in the sample.
Reaction of Fats (pH) ✓ Fatty acids in aqueous state have a pH
below 7
Coconut oil generally have a pH value between
7-8
is used to detect the presence of glycerol and fat.
Acrolein test
Acrolein Formation
Positive result
: If glycerol is present in the sample, it will give a pungent smell.
Saponification Test
Sample + Strong Alkali NaOH + Ethanol)
Saponification Test based on the
saponification reaction
triglycerides of lipids react with an (blank) to produce soap and glycerol in the presence of (blank)
alkali NaOH, ethanol
Saponification Test also known as
alkaline hydrolysis of esters
Saponification Test Result:
Formation of Froth
Sudan IV Test
(Sample + Sudan IV)
detect the presence of lipid in a solution
Sudan IV Test
Sudan IV Test based upon the principle of binding and solubility of (blank) in non-polar compounds
lipid
