Study Set Content:
Sudan IV Test Result
Gives red-orange color to the solution
Liebermann-Burchard Test components
(Crystals of cholesterol + Chloroform + Acetic anhydride + Conc. H2SO4)
Dichromate Test components
Sample + 5% potassium dichromate + Conc. Nitric acid
detect the presence of glycerol
Dichromate Test
based on the principle of an oxidation reaction
Dichromate Test
detect the presence of cholestero
Liebermann-Burchard Test
Cholesterol reacts with the
strong concentrated acid
strong concentrated acid i.e.
sulphuric acid and acetic anhydride
act as a dehydrating and oxidizing agent.
Sulphuric acid and acetic anhydride
Liebermann-Burchard Test result
bluish green color to the solution.
glycerol and dichromate ions react to give a
brown color
Then, the (blank) oxidize the glycerol and reduce into chromous ions by giving a blue color to the solution in the presence of nitric acid.
chronic ions
Dichromate Test Positive Result
If the color of the solution changes from (blank), it indicates glycero
brown to blue
The brown color will not change into blue
Negative resullt
Test for the Free Fatty acids components
(Phenolphalein + Dil. NaOH + Lipid sample
based on the neutralization reaction
Test for the Free Fatty acids
neutralizes by adding free fatty acids into the lipids
alkali
If the pink color disappears by adding the lipid sample
Positive Res
The pink color will not disappear
Negative Result
How does fixed oil, fats, and waxes differ based on their physical and chemical characteristics?
Triglycerides/ Triacylglycerols
Fixed oils
Fats
Waxes
