Study Set Content:
Antigen and antibody
Bioaffinity chromatography or Affinity Chromatography
Enzyme and inhibitor
Bioaffinity chromatography or Affinity Chromatography
hormone and carrier
Bioaffinity chromatography or Affinity Chromatography
Synthetic polymer
SEC
Biopolymer
SEC
receptor and ligand
Bioaffinity chromatography or Affinity Chromatography
Polysaccharide
SEC
protein and nucleic aacids
Bioaffinity chromatography or Affinity Chromatography
Pump
Injector
HPLC Column
Detector
Waste
Data
The most commonly used phase, applicable to
most problems in analysis of pharmaceutical
formulation.
1. Octadecylsilyl silica gel (ODS)
Useful alternative to ODS Phases.
Octyl silane and butyl silane silica gel
Often used in the past for problematical
compounds, but with gradual improvement of
reverse phases.
Silca Gel
Useful for slightly more selective analyses of
compounds containing large numbers of aromatic
rings.
3. Phenyl Silane Silica gel
A moderately polar phase often used for the
analysis of sugars and surfactants. In the case of
sugars the chromatographic interactions are due to
hydrophilic interactions
5. Aminopropyl Silica Gel
Usually based on ion pairing of the analyte with
sulfonic acid groups on the surface of the
stationary phase.
Strong cation exchanger (SCX)
