Study Set Content:
Impregnation of silica, alumna, or ion exchange resins can also be
made.
Impregnated papers:
FACTORS THAT GOVERN THE CHOICE OF PAPER:
a. Nature of the sample and solvent used.
b. Based on the quantitative or qualitative analysis
c. Based on the thickness of the paper.
Pure solvents buffer solutions, pure solvents, or even mixtures of solvents are acceptable.
Mobile phase
Those that have a strong affinity for water.
Hydrophilic mobile phase
These are nonpolar mobile phases that have a decreased affinity
for water.
Hydrophobic mobile phases
Hydrophilic mobile phase
a. Isopropanol: ammonia:water 9:1:2
b. Methanol: water 4:1
c. N-butanol: glacial acetic acid: water 4:1:5
Hydrophobic mobile phases
dimethyl ether: cyclohexane kerosene : 70% isopropanol
The most commonly used solvents are the
polar ones
However, the selection of the solvent is
contingent on the characteristics of the substance that is to be
seperated
Butan-1-ol/Acetic acid/Water Ratio
40/10/50
Butan-1-ol/Pyridine/Water
33/33/33
Methanol/Pyridine/Water
25/12/63
Propan-1-ol/Petroleum ether
4/96
Chloroform/Petroleum ether
30/70
Butan-1-ol/Acetic acid/Water Compounds
Amino Acids
Butan-1-ol/Pyridine/Water
Amino Acids
Methanol/Pyridine/Water
Amino Acids
Propan-1-ol/Petroleum ether
Plant Pigment
Chloroform/Petroleum ether
Plant Pigment
an important component in paper chromatography because it provides
the conditions for the separation process to take place.
Developing Chamber
