Study Set Content:
remains fixed in place while the
stationary phase
carries the components of the mixture through the medium being used.
Mobile phase
acts as a constraint on many of the components in a mixture, slowing them down to move slower than the mobile phase.
stationary phase
Different types of chromatography
Paper chromatography
Thin Layer chromatography
Gas chromatography
High-pressure liquid chromatography
Examples: Gravimetric analysis, titration and bioassays
Chemical procedure or Classical method
assay of volatile oils, assay of alkaloids
Special method
Example: Spectrometry-absorbance; refractometer-refractive index; polarimeter-angle of rotation of light
Instrumental method
Instrumental Method
Types
Optical method
Electrical Method
Types of Optical Method
Based on absorption of radiant energy
Based on emission of radiant energy
Polarimetry
Refractometry
Turbidimetry and Nephelometry
Types of Electrical Method
Polarography
Potentiometry
Coulometry
Chromatrography
Mobile phase
after mobile phase
solvent delivery pump
after solvent delivery pump
sample injector
sample
GPC column
after gpc column
Detector
chromatogram
carrier gas
gas flow regulator
sample
