Study Set Content:
Brine shrimp lethality assay
The mortality endpoint of this bioassay is defined as the absence of ()motion during ()sec of observation
controlled forward , 30
%Death=
Number of dead nauplii/ Number of dead nauplii+Number of live nauplii x 100%
The higher the % of death the greater the
cytotoxicity
is evaluated in vivo in rabbits after they have been shaved. The product is applied to the skin and the appearance of oedema and/or erythema is evaluated at 1, 24, 48, and 72 h after application.
Draize Acute skin irritation
s a laboratory method used to evaluate the potential of chemicals, cosmetics, or pharmaceutical substances to cause irritation or damage to the eyes. It was introduced by Dr. John H. Draize and his colleagues in 1944 and is primarily conducted on rabbits, particularly New zealand (albino rabbits), due to their large, sensitive eyes and ease of handling.
Draize Eye Irritation Test
causing serious or irreversible eye damage that persists for 21 days post-exposure.
Type 1 Irritants
are reversible before 21 days
Type 2 Irritants
are differentiated by the severity of irritation with () irritants more severe than () and further subcategorized if effects are fully reversible within 7 days of substance application.
Type 2A, Type 2B
OECD meaning
ORGANIZATION FOR ECONOMIC COOPERATION AND DEVELOPMENT
OECD TEST GUIDELINE 404
Acute Dermal Irritation/Corrosion
OECD TEST GUIDELINE 430
In Vitro Skin Corrosion: Transcutaneous Electrical Resistance Test Method (TER)
OECD TEST GUIDELINE 435
In Vitro Membrane Barrier Test Method for Skin Corrosion
OECD TEST GUIDELINE 439
In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method
OECD Test No. 406:
Skin Sensitization
OECD Test No. 429:
Skin Sensitization: Local Lymph Node Assay (LLNA)
442 -A
Skin Sensitization: Local Lymph Node Assay: DA
OECD Test No. 442 B
Skin Sensitization: Local Lymph Node Assay: BrdU-ELISA
OECD Test No. 442C
In Chemico Skin Senzitisation Assays
442 -D
In Chemico Skin Sensitisation
OECD 402
Acute Dermal Toxicity
