Study Set Content:
Assays That Are Used
Assay of Furosemide in Tablet form
Assay of cyclizine lactate in injection
Assay of penicillins by derivatisation
Assay of adrenaline in lidocaine (lignocaine) adrenaline injection
Assay of Furosemide in Tablet form A typical example of a straightforward tablet assay is the analysis of furosemide (frusemide) tablets:
Tablet powder containing ca (blank) of furosemide
0.25 g
Tablet powder containing ca 0.25 g of furosemide (frusemide) is
shaken
. Tablet powder containing ca 0.25 g of furosemide (frusemide) is shaken with
300 ml of 0.1 M NaOH
Tablet powder containing ca 0.25 g of furosemide (frusemide) is shaken with 300 ml of 0.1 M NaOH to extract the
acidic furosemide
The extract is then made up to
500 ml
The extract is then made up to 500 ml with
0.1 M NaOH.
A portion of the extract is filtered and(blank)of the filtrate is made up to
5 ml , 250 ml with 0.1 M NaOH.
. The absorbance of the diluted extract is measured at
271nm
The A (1%, 1 cm) value at 271 is (blank) in basic solution.
580
Assay of cyclizine lactate in injection The steps in this assay are more difficult to follow since a number of extractions take place prior to preparing the final dilution, in order to remove excipients
Dilute (blank) of injection to (blank) with (blank)
5 ml, 100 ml, 1 M sulphuric acid.
Add (blank) to 20 ml of this solution and shake with (blank) 50 ml quantities of (blank)
2 g of sodium chloride, two, ether
Add 20 ml of
5 M sodium hydroxide
Add 20 ml of 5 M sodium hydroxide and extract with (blank) 50 ml quantities of ethe
three
(blank) the ether extracts and then wash with two 10 ml quantities of a saturated solution of (blank)
Combine, sodium chloride.
Extract the ether layer with (blank) of 0.05 M sulphuric acid and then with (blank) quantities of water.
two 25 ml quantities, two 10 ml
Combine the (blank) and (blank) extracts and dilute to 100 ml with water.
acidic, aqueous
. Dilute 5 ml of this solution to 200 ml with 0.05 M sulphuric acid and measure the absorbance of the resulting solution at
225 nm
The BP utilises formation of a (blank) in order to quantify penicillins in formulations
derivative
Some penicillins do not have distinctive
chromophores
