Study Set Content:
Phenylalanine
Neutral
Proline
Neutral
Serine
Neutral
Threonine
Neutral
Tryptophan
Neutral
Tyrosine
Neutral
Valine
Neutral
Selenocysteine
(Neutral
Pyrrolysine
Basic
Ninhydrin test positive result
Gives purple colour
Used for the detection of all Alpha-L-amino acids
Ninhydrin test
Is a test for amino acids and proteins with a free - NH2 group.
ninhydrin test
the specific test for the detection of phenolic amino acids like tyrosine
Millon’s Test
When such an -NH2 group reacts with ninhydrin, a
purple-blue complex
Imino acids like proline also reacts with the Ninhydrin and gives a
yellow-colored compound.
Millon's Test positive result
Gives red color
Used for the deteciton of tyrosine amino acid
Millon's test
The principle of Millon’s test is based upon the “(blank)” reaction.
Nitration
Millon’s test makes use of
nitrifying agent
he principle of Millon’s test is based upon the “Nitration” reaction. Millon’s test makes use of nitrifying agent i.e. concentrated nitric acid, by which the phenolic amino acids are converted into nitrated amino acid by giving a
red color to the solution
