Study Set Content:
The overall effect of this conversion is that the –OH group is moved from the
3' to the 4' position on the molecule.
This transformation yields the molecule
isocitrate
Step 3 enzyme involved
Isocitrate Dehydrogenase
The enzyme isocitrate dehydrogenase catalyzes the oxidation of the
–OH group
The enzyme isocitrate dehydrogenase catalyzes the oxidation of the –OH group at the
4' position of isocitrate
to yield an intermediate which then has a (blank) removed
carbon dioxide molecule
The enzyme isocitrate dehydrogenase catalyzes the oxidation of the –OH group at the 4' position of isocitrate to yield an intermediate which then has a carbon dioxide molecule removed from it to yield
α-ketoglutarate.
Reaction 4: enzyme involved
Alpha-ketoglutarate dehydrogenase
An α-ketoglutarate loses a
carbon dioxide molecule
An α-ketoglutarate loses a carbon dioxide molecule and (blank) is added in its place.
coenzyme A
An α-ketoglutarate loses a carbon dioxide molecule and coenzyme A is added in its place. The decarboxylation occurs with the help of
NAD
An α-ketoglutarate loses a carbon dioxide molecule and coenzyme A is added in its place. The decarboxylation occurs with the help of NAD, which is converted to
NADH
The resulting molecule is called
succinyl-CoA.
Reaction 5: enzyme involved
Succinyl-CoA Synthetase
In this step a molecule of (blank) ) is synthesized
guanosine triphosphate (GTP)
. In this reaction, a (blank) first attacks the succinyl-CoA
free phosphate group
n this reaction, a free phosphate group first attacks the succinyl-CoA molecule releasing the
CoA
After the phosphate is attached to the molecule, it is transferred to the
GDP
After the phosphate is attached to the molecule, it is transferred to the GDP to form
GTP
The resulting product is the molecule
succinate
