Study Set Content:
It is faster than paper chroma, better separation, and the choice between different adsorbents.
THIN LAYER CHROMATOGRAPHY
A technique used to isolate (). The experiment is conducted on a sheet of
()which is coated with a thin layer of adsorbent material.
non-volatile mixtures, aluminum foil, plastic, or glass
a simple, quick, and inexpensive procedure that gives the chemist a quick answer as to how
many components are in a mixture.
THIN LAYER CHROMATOGRAPHY
is also used to support the identity of a compound in a mixture when the Rf of a
compound is compared with the Rf of a known compound (preferably both run on the same
TLC plate).
TLC is
one of the easiest and most versatile methods of doing this because of its low cost, simplicity,
quick development time, high sensitivity, and good reproducibility.
Thin layer Chromatography
used by many industries and fields of research, including pharmaceutical production, clinical
analysis, industrial chemistry, environmental toxicology, food chemistry, water, inorganic,
and pesticide analysis, dye purity, cosmetics, plant materials, and herbal analysis.
Thin layer Chromatography
In its simplest form, glass plates are coated with a uniform layer of
silica gel (SiO2).
Thin layer Chromatography aka
planar chromatography.
THIN LAYER CHROMATOGRAPHY Main Principle:
Adsorption
The components will() in solubility and in the strength of their adsorption to the adsorbent and some
components will be carried farther up the plate than others. When the solvent has reached the top of the
plate, the plate is () from the developing chamber, dried, and the separated components of the
mixture are visualized.
differ, removed
used when the stationary phase is polar. Example: Silica gel and the mobile
phase is an organic solvent or a mixture of organic solvents which is less polar than the stationary
phase.
Normal Phase
when the stationary phase is a silica bonded with an organic substrate such as
long chain aliphatic acid..
Reverse Phase
INSTRUMENTATION:
3 Components:
a. TLC Plate
b. TLC Chamber
c. Solvent phase
(Stationary Phase)
TLC Plate
(Mobile Phase)
Solvent phase
These are stable and chemically inert plates, where a thin layer of stationary phase is applied on its whole
surface layer.
PLATES
TLC plates (also known as
chromatoplates)
can be prepared in the lab, but are most commonly
purchased.
TLC plates
are among the most common stationary phases, but others
are available as well.
Silica gel and alumina
Silica Gel Acidic or Basic
Acidic
