Study Set Content:
The principle of separation in GC is
PARTITION
The mixture of component to be separated is converted to vapour and mixed with () mobile phase
gaseous
The component which is more soluble in the stationary phase travels () and eluted later.
Slower
The component which is () soluble in the stationary phase travels faster and eluted out first.
less
No two components have the same () conditions.
partition coefficient
So the components are
separated according to their
partition coefficient.
“the ratio of solubility of a substance distributed between two
immiscible liquids at a constant temperature.”
Partition coefficient
In gas chromatography, the components of a sample are dissolved in a solvent and vaporized
in order to separate the analytes by distributing the sample between two phases:
a stationary
phase and a mobile phase.
The mobile phase is a chemically() that serves to carry the
molecules of the analyte through the heated column.
inert gas
The equilibrium is partitioning, and the components of the sample will partition (i.e. distribute) between
the two phases:
the stationary phase and the mobile phase.
consists of a packed column
The stationary phase
The stationary phase consists of a packed column in which the packing or solid supporting itself acts as
stationary phase (gas-solid
or is coated with the liquid stationary phase such as a high
boiling polymer
(gas-liquid chromatography).
is to a great extent more widely used than gas-solid.
The gas-liquid chromatography
The mobile phase (carrier gas) is comprised of an inert
helium, argon, or nitrogen.
It acts as the
carrier gas that serves to carry the molecules of the analyte through the
column.
During the separation, the components of a sample are dissolved in a solvent and () in order to
separate the analytes.
Vaporized
The separation of compounds is based on the different strengths of interaction of the compounds with
the stationary phase what rule
(“like-dissolves-like”
The component which is more soluble in the stationary phase travels () and eluted ()
slower, later
The component which is less soluble in the stationary phase travels () and eluted out ()
faster, first.
